Molecular Embryology: Methods and Protocols by Paul T. Sharpe (Editor), Ivor Mason (Editor) free book download

Molecular Embryology: Methods and Protocols
by Paul T. Sharpe (Editor), Ivor Mason (Editor)

Product Details
* Hardcover: 756 pages
* Publisher: Humana Press; 1st edition (January 15, 1999)
* Language: English
* ISBN-10: 0896033872
* ISBN-13: 978-0896033870

Table of Contents

Part I. Mouse Embryo

The Mouse as a Developmental Model
Paul T. Sharpe

Culture of Postimplantation Mouse Embryos
Paul Martin and David L. Cockroft

Organ Culture in the Analysis of Tissue Interactions
Irma Thesleff and Carin Sahlberg

Treatment of Mice with Retinoids In Vivo and In Vitro: Skeletal Staining
Gillian M. Morriss-Kay

Cell Grafting and Labeling in Postimplantation Mouse Embryos
Gabriel A. Quinlan, Paul A. Trainor, and Patrick P. L. Tam

Production of Transgenic Rodents by the Microinjection of Cloned DNA Into Fertilized One-Celled Eggs
San Ling Si-Hoe and David Murphy

Cre Recombinase Mediated Alterations of the Mouse Genome Using Embryonic Stem Cells
Anna-Katerina Hadjantonakis, Melinda Pirity, and András Nagy

Gene Trapping in Mouse Embryonic Stem Cells
Jane Brennan and William C. Skarnes

Production and Growth of Conditionally Immortal Cell Lines from the H-2KbtsA58 Transgenic Mouse
Mark Noble

Reporter Genes for the Study of Transcriptional Regulation in Transgenic Mouse Embryos
Jonathan D. Gilthorpe and Peter W. J. Rigby

Application of lacZ Transgenic Mice to Cell Lineage Studies
Paul A. Trainor, Sheila X. Zhou, Maala Parameswaran, Gabriel A. Quinlan, Monica Gordon, Karin Sturm, and Patrick P. L. Tam

Mouse Primordial Germ Cells: Isolation and In Vitro Culture
Patricia A. Labosky and Brigid L. M. Hogan

Part II. Chicken Embryo

The Avian Embryo: An Overview
Ivor Mason

Chick Embryos: Incubation and Isolation
Ivor Mason

New Culture
Amata Hornbruch

Grafting Hensen's Node
Claudio D. Stern

Grafting of Somites
Claudio D. Stern

Manipulating the Chick Embryo: Notochord Grafts
Andrew Lumsden and Susanne Dietrich

Transplantation of Avian Neural Tissue
Sarah Guthrie

Grafting of Apical Ridge and Polarizing Region
Cheryl Tickle

Tissue Recombinations in Collagen Gels
Marysia Placzek and Kim Dale

Quail–Chick Chimeras
Marie-Aimée Teillet, Catherine Ziller, and Nicole M. Le Douarin

Using Fluorescent Dyes for Fate Mapping, Lineage Analysis, and Axon Tracing in the Chick Embryo
Jonathan D. W. Clarke

Part III. Amphibian Embryos

An Overview of Xenopus Development
C. Michael Jones and Jim Smith

Mesoderm Induction Assays
C. Michael Jones and Jim Smith

Experimental Embryological Methods for Analysis of Neural Induction in the Amphibian
Ray Keller, Ann Poznanski, and Tamira Elul

A Method for Generating Transgenic Frog Embryos
Enrique Amaya and Kristen L. Kroll

Axolotl/newt
Malcolm Maden

Part IV. Zebrafish

The Zebrafish: An Overview of Its Early Development
Nigel Holder and Qiling Xu

Small-Scale Marker-Based Screening For Mutations in Zebrafish Development
Peter D. Currie, Thomas F. Schilling, and Philip W. Ingham

Transgenic Zebrafish
Trevor Jowett

Microinjection of DNA, RNA, and Protein into the Fertilized Zebrafish Egg For Analysis of Gene Function
Nigel Holder and Qiling Xu

Retinoids in Nonmammalian Embryos
Malcolm Maden

Part V. Nonvertebrate Chordates

Protochordates
Peter W. H. Holland and Hiroshi Wada

Part VI. Retrovirus

Gene Transfer to the Rodent Embryo by Retroviral Vectors
Grace K. Pavlath and Marla B. Luskin

Gene Transfer in Avian Embryos Using Replication-Competent Retroviruses
Cairine Logan and Philippa Francis-West

Part VII. Molecular Techniques

Subtractive Hybridization and Construction of cDNA Libraries
Bruce Blumberg and Juan Carlos Izpisúa Belmonte

Differential Display of Eukaryotic mRNA
Antonio Tugores and Juan Carlos Izpísua Belmonte

RT-PCR on Embryos Using Degenerate Oligonucleotide Primers
Anthony Graham

Single-Cell RT-PCR cDNA Subtraction
Damian L. Weaver, César Núñez, Clare Brunet, Victoria Bostock, and Gerard Brady

In Situ Hybridization of Radioactive Riboprobes to RNA in Tissue Sections

Radma Mahmood and Ivor Mason. In Situ Hybridization to RNA in Whole Embryos
Huma Shamim, Radma Mahmood, and Ivor Mason

Wholemount In Situ Hybridization to Xenopus Embryos
C. Michael Jones and James C. Smith

Wholemount In Situ Hybridization to Amphioxus Embryo
Peter W. H. Holland

In Situ Hybridization to Sections (Nonradioactive)
Maria Rex and Paul J. Scotting

Immunohistochemistry Using Polyester Wax
Andrew Kent

Immunohistochemistry on Whole Embryos
Ivor Mason

Whole Embryo Assays for Programmed Cell Death
Anthony Graham

Gene Interference Using Antisense Oligodeoxynucleotides on Whole Chick Embryos: Optimal Ring and Roller-Bottle Culture Technique
Jonathan Cooke and Alison Isaac

Protein Techniques: Immunoprecipitation, In Vitro Kinase Assays, and Western Blotting
David I. Jackson and Clive Dickson

Part VIII. Microscopy and Photography

Microscopy and Photomicrography Techniques
Richard J. T. Wingate

Index

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